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Image Search Results
Journal: Clinical, Cosmetic and Investigational Dermatology
Article Title: A Topical Chinese Herbal Alleviates Psoriasis by Regulating Keratinocytes Pyroptosis Through Inhibition of NLRP3 Inflammasome Activation
doi: 10.2147/CCID.S559712
Figure Lengend Snippet: ( A – C ) mRNA levels of NLRP3, Caspase-1, GSDMD in NHEK cells, n = 3. ( D – I ) Protein levels of NF-κB, P65, NLRP3, caspase-1, GSDMD in NHEK cells, n = 3.*P < 0.05, **P < 0.01, ***P < 0.001 compared with Control; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with Model; & P < 0.05, && P < 0.01, &&& P < 0.001 compared with QS; ^P < 0.05, ^^P < 0.01 compared with Mcc950.
Article Snippet: The sections were then incubated overnight at 4°C with the following primary antibodies:
Techniques: Control
Journal: BMC Biology
Article Title: Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
doi: 10.1186/s12915-023-01550-0
Figure Lengend Snippet: Rapid induction of IRF1 transcription is mediated by PKC-NF-kB signaling. A PKC activity levels in chicken embryos treated with cold stimulation (28°C) for 12 hours, N = 7. B PKC activity levels in cESCs treated with cold stimulation (28°C) for 3 hours, N = 7. C Immunostaining of cESCs with or without 3 hours of cold stimuli. Staining was performed with an antibody against PKC (green). DAPI was applied as a counterstain (blue). Scale: 20 μm. D NF-kB luciferase levels in cESCs treated with cold stimulation (28°C), a PKC activator (TPA) or a PKC inhibitor (GO 6983) for 3 hours. cESCs transfected with an NF-kB luciferase reporter vector and untransfected cESCs served as the control group (Ctrl) and negative control group (NC), respectively, N = 3. E Immunostaining of cESCs with or without 3 hours of cold stimuli. Staining was performed with an antibody against P65 (green) or DAPI (blue). Scale: 20 μm. F Changes in gene expression after treatment with cold stimuli, and an activator and/or inhibitor of PKC and NF-kB for 3 hours in cESCs, N = 4. The mean ± SEM is shown in all panels. * p < 0.05; ** p < 0.01; *** p < 0.001. N indicates biological replicates
Article Snippet: The sections were incubated overnight at 4°C with primary antibodies against H3S10P (Cat: 3377T; CST; 1:200), PCNA (Cat: abs120180; Absin, Shanghai, China; 1:200), PKC (Cat: NB600-201; Novus, Littleton, CO, USA; 1:100) or
Techniques: Activity Assay, Immunostaining, Staining, Luciferase, Transfection, Plasmid Preparation, Control, Negative Control, Gene Expression